84-1073-62　Myostatin Propeptide 50ug　M9875-03

特徴

• Myostatin (GDF 8) is expressed uniquely in human skeletal muscle as a 12kD mature glycoprotein consisting of 109 aa residues and secreted into plasma.
• Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass.
• Studies have shown that myostatin could play an important role in cardiac development and physiology.
• In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene.
• The myostatin propeptide is known to bind and inhibit myostatin in vitro.
• This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide.
• The myostatin propeptide is negative regulator of myostatin in vivo.
• Applications:Suitable for use in ELISA, immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: IndirectOptimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
• Amino Acid Sequence:MGNENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.