Myostatin (GDF 8) is expressed uniquely in human skeletal muscle as a 12kD mature glycoprotein consisting of 109aa residues and secreted into plasma.
Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass.
Studies have shown that myostatin could play an important role in cardiac development and physiology.
In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene.
The myostatin propeptide is known to bind and inhibit myostatin in vitro.
This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide.
The myostatin propeptide is negative regulator of myostatin in vivo.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommened Dilutions:Optimal dilution to be determined by researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：bovine
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Myostatin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human Myostatin. The immunization antigen (16.7kD) contains 152 amino acids (aa267-375) of Recombinant human Myostatin. N-terminal His-tag including the spacer 43aa.
