The fasting-induced adipose factor (FIAF, ANGPTL4, PGAR, HFARP) was previously identified as a novel adipocytokine that was up-regulated by fasting, by peroxisome proliferators-activated receptor agonists, and by hypoxia.
FIAF and other adipocytokines including resistin, adiponectin, acylationstimulating protein, plasminogen activator inhibitor-1, rennin angiotensin system, metallothioneins and tumor growth factor-b have been implicated in a variety of different processes, ranging from blood pressure control to lipid metabolism and insulin senziting.
It has been tempting to attribute many of the clinical abnormalities associated with obesity from insulin resistance to altered secretion of particular adipocytokines.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: IndirectImmunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human FIAF.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to 220aa of human FIAF with a N-terminal His-tag (16aa). MW of Antigen: 25kD. Sequence Homology: human ANGPTL4 precursor (aa26-229) without signal sequence: 100%.
