84-1073-51　Agouti-Related Protein (AGRP) 50ug　A1059-63A

特徴

• The Agouti-related protein (Agrp) -- secreted hypothalamic factors regulated by the metabolic state in mammals -- is involved in energy homeostasis and plays an important role in the control of feeding behavior.
• Agouti protein and Agouti-related protein are paracrine-signaling molecules that normally regulate pigmentation and body weight.
• The mature human protein is a single polypeptide chain of 112 amino acid residues consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain.
• Circular dichroism studies indicated that the purified protein contains primarily random coil and beta-sheet secondary structure.
• The hypothalamic expression of Agrp is regulated by leptin, and overexpression of Agrp in transgenic animals results in obesity and diabetes.
• Applications:Suitable for use in Indirect ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Amino Acid Sequence;MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.