84-1073-50　Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28) 100ug　A0891-01

特徴

• Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene which is physiologically active and specifically and highly expressed in adipose cells (adipokine)
• The protein belongs to the soluble defence collagen superfamily.
• It has a collagen-like domain which is structurally homologous with collagen VIII and X and complement factor C1q-like globular domain.
• Adiponectin forms homotrimers, which are the building blocks for higher-order complexes found circulating in serum.
• A reduction in adiponectin serum levels is accompanied by insulin resistance states such as obesity and type 2 diabetes mellitus.
• It is also reported in patients with coronary artery disease.
• Increased adiponectin levels are associated with type 1 diabetes mellitus, anorexia nervosa and chronic renal failure
• Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation.
• It inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Indirect ELISA: >1:100,000 using 2.5ng of antigen coated per wellWestern Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.