Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue.
Full-length adiponectin was expressed in HEK293 cells stably transfected with pcdm-F vector encoding FLAG epitope-tagged mouse adiponectin.
Recombinant adiponectin was purified using anti-FLAG M2 affinity gel (Sigma-Aldrich) and was eluted with FLAG peptide.
FLAG peptides were removed by an extensive wash with an excessive volume of saline.
Highly sensitive matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass-spectrometric analysis confirmed that FLAG peptide was not detectable in the protein solution.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Indirect ELISA: >1:100,000 using 25ng of antigen coated per well
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2
Specificity：The recombinant mouse adiponectin is 100% homologous with the Mouse serum adiponectin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant Murine Adiponectin produced in HEK293 (Human embryonic kidney cell line).
