Recommended Dilution:Western Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells.
An additional unknown band was detected at Mr 97kD in the lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity：Recognizes human Smad2 Mr 55-60kD and Smad3 Mr 50kD as determined by using Smad2 and Smad3 transfected Cos cells. Species Crossreactivity: Predicted to crossreact with mouse and rat based on conservation of immunogen sequence.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Fusion protein corresponding to amino acids 186-273 of human Smad2.
