A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway.
Activation of c-Raf is the best understood, and involves phosphorylation at multiple activating sites, including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499.
p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity.
Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and BRaf (Ser445), although this site is constitutively phosphorylated in B-Raf.
Inhibitory 14-3-3 binding sites (Ser259 and Ser621) on c-Raf can be phosphorylated by Akt and AMPK, respectively.
While A-Raf, BRaf, and c-Raf are similar in sequence and function, differential regulation has been observed.
Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to c-Raf Tyr341.
The B-Raf mutation V599E results in elevated kinase activity and is commonly found in malignant melanoma.
Applications:Suitable for use in Western Blot Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human c-Raf protein. Species Crossreactivity: mouse, monkey and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to a region surrounding Pro302 of human c-Raf.
