Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2).
PKC isoforms have been classified into three groups: classical, novel and atypical PKCs.
Members of these three groups contain a pseudosubstrate or autoinhibitory domain with substrate binding sites in the catalytic domain, preventing its activation in the absence of cofactors or activators.
Other members have recently been added to the PKC superfamily based on homology within the catalytic domain.
PKCμ, or PKD, is regulated by DAG and TPA through its C1 domain.
However, PKD is distinguished by a transmembrane domain and PH domain, as well as by its unique substrate recognition and Golgi localization.
The PKC-related kinases lack a C1 domain and thus do not respond to DAG or phorbol esters.
Instead, they are activated by phosphatidylinositol lipids, and their kinase activity is directly regulated by GTPases through Rho binding to the homology region 1 (HR1).
The activity of PKC is under the control of three distinct phosphorylation events.
Specifically, Thr500 at the activation loop, the Thr641 autophosphorylation site and the Ser660 hydrophobic site at the carboxy terminus of PKCb II are phosphorylated in vivo (2).
For the atypical PKC isoforms, there is no phosphorylation in the hydrophobic region, which has a glutamic acid residue in place of the serine or threonine residue found in other PKC isoforms.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:25Western Blot: 1:1000Immunoprecipitation: 1:50Immunofluorescence (IC): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot.
Store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total PKCa protein. Does not cross-react with endogenous levels of other PKC isoform MW: 80kD. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：FC IC IF IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human PKCa.
