84-1073-35　NOS III, phosphorylated (Ser113) (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial) 100ul　N5350-28H

特徴

• Endothelial nitric-oxide synthase (eNOS is an important enzyme in the cardiovascular system.
• It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2).
• eNOS is regulated by phosphorylation at multiple sites.
• The two most thoroughly studied sites are the activation site serine 1177 and the inhibitory site threonine 495 (3).
• Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating serine 1177 in response to various stimuli (4,5).
• In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting threonine 495 dephosphorylation (6,7).
• eNOS is activated by VEGF, and this activation is associated with dephosphorylation of eNOS at serine 113.
• Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot.
• Store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.