Microtubule associated proteins regulate the stability of microtubules, controlling processes such as cell polarity/differentiation, neurite outgrowth, cell division, and organelle trafficking (1).
The MARK MAP/microtubule affinity-regulating kinases) family MARK1-4) of serine/threonine kinases were identified based on their ability to phosphorylate microtubule associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6).
MARKs phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4).
In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer’s disease.
Over-expression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4).
The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human MARK4 protein at ~79kD. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the sequence surrounding Cys514 of human MARK4.
