Cyclin E1 and cyclin E2 can associate with and activate cdk2.
Upon DNA damage, upregulation/ activation of the cdk inhibitors p21Waf1/Cip1 and p27Kip1 prevents cyclin E/cdk2 activation, resulting in G1/S arrest.
However, when conditions are favorable for cell cycle progression, cyclin D/cdk4/6 phosphorylates Rb and is thought to reduce the activity of p21Waf1/Cip1 and p27Kip1, allowing subsequent activation of cyclin E/cdk2.
Cyclin E/cdk2 then further phosphorylates Rb to allow progression into S-phase, where cyclin E/cdk2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis.
Turnover of cyclin E is largely contolled by phosphorylation resulting in SCFFbw7-mediated ubiquitination and proteasomedependent degradation.
Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, Cdk2 and GSK3.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1:50Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:100 Citrate/TBSTImmunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human cyclin E only when phosphorylated at threonine 62 (cyclin E1 isoform 2) or threonine 77 (cyclin E1 isoform 1).
Isotype：IgG
Calc Applications Abbrev：FC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phospho-peptide corresponding to residues surrounding Thr62 of human cyclin E.
