Rac1 and cdc42 are members of the Rho-GTPase family.
They play key roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development.
It has been established that GTP binding stimulates the activity of Rac1/cdc42.
A putative Akt phosphorylation site at Ser71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay (1).
Phosphorylation at this site may inhibit GTP binding of Rac1, attenuating the signal transduction pathway downstream of Rac1 (1).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Cdc42 protein. The antibody does not cross-react with other GTPases. Species Crossreactivity: monkey, mouse, rat and bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mk Mo Rt
Immunogen：Synthetic peptide (KLH-coupled) corresponding to residues surrounding Lys135 of human Cdc42. Species sequence homology: chicken, canine, Xenopus, and zebrafish
