Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) is one of the key executioners of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1).
Activation of Caspase 3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments.
Cleavage of Caspase 3 requires aspartic acid at the P1 position (2).
Applications:Suitable for use in FLISA, Flow Cytometry, Immunocytochemistry and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:Flow Cytometry: 1:50Immunofluorescence (IF-IC): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt at 4°C.
Freezing Alexa Fluor® conjugates will result in a substantial loss of activity.
Alexa Fluor® conjugates are sensitive to light.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, <0.09% sodium azide. Labeled with Alexa Fluor®488. F/P Ratio: 2:6. Absorption: 494nm. Emission: 520nm.
Specificity：Recognizes endogenous levels of the large fragment (17/19kD) of activated human Caspase 3 resulting from cleavage adjacent to aspartic acid 175. Does not recognize full length Caspase 3 or other cleaved caspases. Species Crossreactivity: mouse, monkey and rat*. *Note: Species Crossreactivity other than human is determined in Western Blots with the unconjugated form of this antibody.
Isotype：IgG
Calc Applications Abbrev：FC FLISA IC IF
Calc Crossreactivity：Bo Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to N-terminal residues adjacent to Asp175 of human Caspase 3. Species Sequence Homology: Porcine, bovine and canine
