Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway.
The 265kD ACCa is the predominant isoform in liver, adipocytes and mammary gland, while the 280kD ACCb is the major isoform in skeletal muscle and heart.
Phosphorylation by AMPK at Ser79, or by PKA at Ser1200, inhibits the enzymatic activity of ACC.
ACC is a potential target of anti-obesity drugs.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunohistochemistry (Paraffin): 1:50.
Antigen retrieval with citrate buffer is recommended.
Immunoprecipitation: 1:50Immunofluorescence (IC): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of all isoforms of human acetyl CoA carboxylase protein at ~280kD. Species Crossreactivity: bovine, mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：FC IF IHC IP WB
Calc Crossreactivity：Bo Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Ser523 of human acetyl CoA carboxylase a1. MW of Antigen: 280kD. Species Sequence Homology: chicken and Drosophila 100%.
