Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types.
These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization.
WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed.
These proteins have 48% identity in human with higher homology in the functional regions of these proteins.
Phosphorylation at serine and tyrosine residues regulates the activity of both proteins.
WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets.
Phosphorylation of the analogous site in N-WASP (Tyr-256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:10,000 ELISA: 1:20,000Immunocytochemistry: 1:100Optimal dilutions to be determined by the researcher.
Positive Controls:Pervanadate treated A431, HeLa and endothelial cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Specificity：Recognizes a 65kD protein corresponding to the molecular mass of human phosphorylated N-WASP. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at a higher MW after pervanadate treatment, may be due to weak cross reactivity with phosphotyrosine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acid residues around phosphorylated tyrosine 256 of human N-WASP, coupled to BSA. Sequence homology: Two amino acid sequence difference in the region surrounding WASP tyrosine 291.
