The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion.
At least eleven isozymes have been described.
PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V).
The Nterminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain.
The conventional PKC subfamily (a, bI, bII, and g) is regulated by both Ca2+ and diacylglycerol.
The PKC pathway represents a major signal transduction system that is activated following ligandstimulation of transmembrane receptors by hormones, neurotransmitters and growth factors.
The phosphorylation of multiple sites in conventional PKCs regulates their activity.
In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCbI and PKCa, respectively.
This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
Western blot analysis of immunoprecipitates from neonatal rat brain lysate using an anti-PKCa antibody.
Control and alkaline phosphatasetreated precipitates were probed with anti-PKCa and anti-phospho-PKCa (Ser-657/Tyr-658).
Thelatter shows no detection of PKCa after phosphatase treatment.
Applications:Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Specificity：Recognizes human Protein Kinase C, phosphorylated at Ser-657/Tyr-658. Detects an 82kD protein corresponding to the molecular mass of phoshorylated PKCa on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were seen in other PKCa-expressing cells, such as human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCa and PKCb, but not other PKC isoforms in rat brain lysate.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rb Rt
Immunogen：Phospho-PKCa (Ser-657/Tyr-658) synthetic phosphopeptide (coupled to carrier protein) corresponding to amino acid residues around serine 657 and tyrosine 658 of human PKCa. This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition, this dual site is homologous to threonine 674 and tyrosine 675 in human PKCg, although this reactivity has not been confirmed.
