ß-Catenin is a 92kD protein that binds to the cytoplasmic tail of E-Cadherin.
The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions.
Deletions in the cytoplasmic domain of ECadherin eliminate catenin binding and result in a loss of cell adhesion.
Tyrosine phosphorylation of ß-Catenin can regulate its interaction with critical components of adherens junctions.
Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro.
Overexpression of these kinases in epithelial cells disrupts interactions between a- and ß-Catenins.
The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of ß-Catenin.
Src family kinases can also phosphorylate tyrosine 654 in theC-terminal armadillo repeat of ß-Catenin.
This phosphorylation regulates ß-Catenin binding to E-cadherin.
Thus, site-specific tyrosine phosphorylation of ß-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation or for antigen applications in immunological protocols.
Other applications not tested.
Recommended Dilutions:Western blotting 1:1000.
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1hour at room temperature
Immunoprecipitation: 5-10ulELISA: 1:2000Optimal dilutions to be determined by the researcher.
Control Peptide:C2069-54A Catenin, beta, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Specificity：Detects a 92kD protein corresponding to the molecular mass of ß-Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells. Similar results were seen in human endothelial, A431, and HeLa cells.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：ß-Catenin synthetic peptide (coupled to carrier protein) corresponding to amino acid residues in the N-terminal region of human ß-Catenin. This human sequence is highly conserved in rat and mouse ß-Catenins.
