Akt, also known as Protein Kinase B (PKB) and Rac (Related to the A and C kinases), is a 60kD serine/threonine kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis.
Akt phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1.
Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner.
Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of Akt coincident with its phosphorylation and activation.
Upon activation, Akt associates with members of the PKC family of kinases, such as PKCd and PKCz.
Ceramide-activated PKCz leads to phosphorylation of the Akt-Pleckstrin Homology (PH) domain on threonine 34.
This phosphorylation inhibits PIP3 binding to Akt, preventing activation of the kinase by insulin.
Since Akt confers a prosurvival signal and regulates pathways in response to insulin, suppressing its activation may be one mechanism by which ceramide promotes cell death and induces insulin resistance.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature
ELISA: 1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Specificity：Recognizes a 60kD protein corresponding to the apparent molecular mass of phosphorylated Akt on SDS-PAGE immunoblots of A431+ calyculin A cell lysate. Similar results were seen in calyculin A treated human aortic endothelial and HeLa cells, rabbit spleen fibroblasts, and rat pituitary cells.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rb Rt
Immunogen：Phospho-Akt (Thr-34) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around threonine 34 of human Akt. This sequence is highly conserved in rat and mouse Akt.
