Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes.
The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes.
Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.
Applications:Suitable for use in ELISA, Western Blot, Neutralization, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:2000 with using 100ng of immobilized gp120 coated on the plate
Western Blot: 1-2ug/mlImmunohistochemistry: 1:200Optimal dilutions to be determined by the researcher.
Biological Activity:Neutralizes HIV-1 as determined by syncycium assays using infected CD4 bearing cells and human PBL co-cultures.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after reciept.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography to ≥95% as determined by SDS-PAGE, reduced.
Form：Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
Specificity：Recognizes native and recombinant HIV-1 gp120/gp160. Does not cross react with other human or animal proteins.
