HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body.
These cells are equipped with receptors by which they bind and endocytose the virus.
The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response
Applications:Suitable for use in Dot Blot, Flow Cytometry, Immunohistochemistry and Direct Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Dot Blot: 1:100Direct Immunofluorescence: 1:50Optimal dilutions to be determined by the researcher.
F/P Ratio:2-3
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50Tests
Host：rabbit
Grade：Highly Purified
Purity：Purified (≥ 95%) by gel exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No stabilizer or preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes native and recombinant HIV-1 gp120 in Dot Blot assays and stains cell surfaces in direct immunofluorescence assays.
