Biological Activity:Biotin conjugated Rabbit anti-gp120 antibody binds to gp120 in CD4/gp120 capture ELISA and Western ELISA.
Applications:A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1.
ELISA and Western ELISA may be performed at dilutions of 1:2000 depending on the concentration of gp120 in the test system.
CD4/gp120 capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand CD4 concentration and gp120 concentration in the test sample.
Typical assays with 100ng of solid phase CD4 will detect at least 10ng/ml gp120.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 2.5% BSA, 0.01% sodium azide. Labeled with Biotin.
Specificity：Binds to native and recombinant gp120 (V3 loop specific) in ELISA and Western ELISA.
