HIV-1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body.
These cells are equipped with receptors by which they bind and endocytose the virus.
The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
Applications:Suitable for use in ELISA, Western Blot, Neutralization, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: ELISA: Yields titers of >1:2000 with 100ng of immobilized gp120 under standard ELISA conditions.
Western Blot: 1-2ug/ml depending on the nature and quantity of the immobilized antigen.
Immunohistochemistry: 1:200Optimal dilutions to be determined by the researcher.
Biological Activity:Neutralizes HIV-1 as determined by syncycium assays using infected CD4 bearing cells and human PBL co-cultures.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography (≥95% SDS-PAGE, reduced)
Form：Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
Specificity：Recognizes native and recombinant HIV-1 gp120/gp160 in ELISA and Western Blot.
