Applications:Suitable for Western Blot, ELISA, Immunoprecipitation, Conjugation and most immunological methods.
Other applications not tested.
Recommended Dilutions:Western Blot: 1;500-1:5000ELISA: 1:5000-1:32,000; 1ug of Sucrose Phosphorylase was used in a standard sandwich ELISA with I1904-46Q and ABTS (A0030), as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. Reconstitute withh 100ul sterile ddH2O.
Specificity：Recognized Sucrose Phosphorylase. Assay by IEP resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Sucrose Phosphorylase.
