Applications:Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry as well as other antibody based assays using streptavidin or avidin conjugates.
Other applications not tested.
Recommended Dilution:ELISA: 1:25,000-1:110,000; 1ug of Sucrose Phosphorylase was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per Goat IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
Specificity：Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase (E. coli).
