Sprouty is a suppressor of EGF and FGF signaling that appears to have a universal inhibitory role on tyrosine kinase signaling.
Evidence suggests that it does this by binding adapter proteins and GAP, thus inactivating Ras.
Sprouty expression is induced by the same pathways that it down-regulates, suggesting it plays a role in an auto-feedback loop.
Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development.
Four isoforms of mammalian sprouty are known (Spry1-Spry4).
The role of the mammalian analogs has not beenclearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation.
Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein.
Significant sequence divergence occurs at the N-terminus.
Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:5000; A 38kD band corresponding to human Sprouty-2 is observed.
Detection occurs using HRP Goat anti-Rabbit IgG and ECL.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Reacts with human Sprouty-2 protein (38kD). Species Crossreactivity: BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan.
