84-1072-68　RAD9, phosphorylated (Ser1129) 100ug　R0444-69

特徴

• Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1.
• DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9.
• Cells have evolved multiple strategies for tolerating genomic damage.
• The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions.
• Another cellular strategy is to delay cell-cycle transitions at multiple points.
• The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G 2/M arrest after irradiation with X-rays.
• Subsequently, it has become clear that Rad9 also functions at the G 1/S, intra-S and mid-anaphase checkpoints.
• Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation.
• Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR).
• Rad9 may also have a pro-apoptotic function.
• This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells.
• Overexpression of Bcl-2 in S.
• pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation.
• Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphosphorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate.
• Mature yeast Rad9 is reported to have an apparent molecular weight of ~148kD.
• The human homolog is reported at 48.5kD.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:5000.
• 0.1ug of phosphorylated peptide (pS1129) was used in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at RT.
• Optimal dilutions to be determined by the researcher.
• Protein Sequence: Saccharomyces cerevisiae 1 msgqlvqwks spdrvtqsai kealhsplad gdmnemnvpv dplenkvnst niiegspkan 61 pnpvkfmnts eifqkslgll desprhddel nievgdndrp nanilhnert pdldrianff 121 ksnrtpgken lltkyqssdl edtplmlrkk mtfqtptdpl eqktfkklks dtgfcyygeq 181 ndgeenasle vteadatfvq maersadnyd calegivtpk rykdelsksg gmqdervqkt 241 qimisaespn sissydknki tgngrttrnv nkvfnnnedn igaieeknpv kkksenyssd 301 dlrernnqii qsneseeine leknlnvsgr endvnnldid insavsgtps rnnaeeemys 361 sesvnnreps kkwifryskd ktennsnrst qivnnprtqe mpldsisidt qplsksfnte 421 tnneletqii vsslsqgisa qkgpvfhstg qteeiktqii nspeqnalna tfetpvtlsr 481 infepilevp etsspskntm skpsnsspip kekdtfnihe revetnnvfs ndiqnssnaa 541 trddiiiags sdfneqkeit driylqlsgk qisdsgsdet ermspneldt kkestimsev 601 eltqelpeve eqqdlqtspk klvveeetlm eikkskgnsl qlhddnkecn sdkqdgtesl 661 dvaliehesk gqsselqknl mqlfpsesqe iiqnrrtikr rqkdtieige eeenrstkts 721 ptkhlkrnsd ldaasikrep scsitiqtge tgsgkdskeq syvfpegirt adnsflskdd 781 iifgnavwcq ytwnykfypg illevdtnqd gcwiyfetgr sltkdediyy ldirigdavt 841 fdgneyvvvg lecrshdlni ircirgydtv hlkkknasgl lgkrtlikal ssisldlsew 901 akrakiiled neknkgdayr ylrhpirgrk smtnvlspkk htddekdint htevynneie 961 sssekkeivk kdsrdalaeh agapsllfss geirtgnvfd kcifvltslf enreelrqti 1021 esqggtvies gfstlfnfth plakslvnkg ntdnirelal klawkphslf adcrfaclit 1081 krhlrslkyl etlalgwptl hwkfisacie kkrivphliy qyllpsgesf rlsldspskg 1141 giiksnnifs fytqflrgsn lrdqicgvkk mlndyivivw grseldsfvk fafaclsagr 1201 mltidlpnid vddtepllna ldslvprigs elsnrklkfl iyanenngks qmkllerlrs 1261 qislkfkkfn yifhteskew liqtiinedt gfhdditdnd iyntisevr
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.