84-1072-66　［取扱停止］RAD9 (Cell Cycle Checkpoint Control Protein RAD9A, DNA Repair Exonuclease Rad9 Homolog A, hRAD9, RAD9 (S pombe) Homolog, RAD9 Homolog, RAD9 Homolog A, RAD9A) 100ug　R0444-67

特徴

• Cells respond to DNA damage by activating a network of signaling pathways that control cellcycle progression and DNA repair.
• Cell cycle checkpoints are mechanisms that transiently delay cell cycle progression when DNA is damaged or DNA replication is incomplete.
• In the fission yeast Schizosaccharomyces pombe, a group of six such checkpoint control genes have been identified and include rad1+, rad3+, rad9+, rad17+, rad26+ and hus1+.
• Mutations in any oneof these genes render cells sensitive to gamma-rays, UV light or the DNA synthesis inhibitor hydroxyurea (HU) and eliminate the ability of cells to delay entry into mitosis after treatment with these agents.
• All of these genes apparently link abnormal DNA structures to cell cycle control.
• As for cell cycle-related genes in general, these checkpoint control genes are highly conserved throughout evolution.
• Human and mouse versions of several of the S.pombe genes have been isolated, providing strong evidence that checkpoint control mechanisms are also highly conserved.
• In mammals, these genes are thought to maintain genomic stability, especially in the presence of DNA damage.
• Therefore, when these genes are altered, genomic instabilitymay occur and lead to cancer.
• The biochemical activities of most of the checkpoint control gene products are not well established, although progress has been made towards learning more about their function.
• For example, examination of the structure of the protein encoded by human or S.pombe rad9 reveals a BH3-like domain in the N-terminal region that can bindthe anti-apoptotic proteins Bcl-2 and Bcl-XL.
• Furthermore, overexpression of the gene from either organism in human cells can cause apoptosis in a BH3 domain-dependent manner.
• Both S.pombe and human versions of the protein can bind two other checkpoint control proteins, Hus1p (HUS1p) and Rad1p (RAD1p).
• Human RAD9 protein binds HUS1 and RAD1 proteins at its C-terminal region, suggesting that RAD9 has at least two functional domains, one involved in apoptosis and the other in cell cycle checkpoint control.
• Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p; is hyperphosphorylated by Mec1p and Tel1p; and is a potential Cdc28p substrate.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:6000 -1:60,000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.