Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs).
Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand.
Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids.
It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements.
PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids.
Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels.
PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids.
Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis.
PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Applications:Suitable for use in ELISA and Western Blot.
Suggested to work Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:4000-1:40,000Western Blot: 1:1000-1:3000 detects a band at ~55kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes PPAR gamma2 Species Crossreactivity: mouse. No reactivity is expected against other subtypes of PPAR.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to MGETLGDSPIDPESDS near the N-terminus of human PPAR, gamma 2. Species sequence homology: canine and porcine 94% (15/16aa), mouse 97%.
