84-1072-63　PPARg1,2 (Peroxisome Proliferator Activated Receptor, gamma) 100ug　P5505-09

特徴

• Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention.
• This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones.
• Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs.
• Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand.
• Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes.
• There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma.
• Mostly target genes are involved in the catabolism of fatty acids.
• Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids.
• PPAR-gamma may also be involved in adipocyte differentiation.
• It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:8000-1:32,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.