Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G1 or S phase and is associated with the phosphorylation of cyclin B1.
Phosphorylation of Plk1 occurs on Thr210 for activation.
Plk1 is involved in the mitotic spindle and mitosis and is associated with the phosphorylation of cyclin B1.
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Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression.
Plk-1 has a nuclear localization and accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence Microscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:3000-1:12,000Western Blot: 1:200-1:2000 (~68kD)Immunohistochemistry: 1:200-1:1000 on kidney, liver, cancer, thyriod, and lymphocyte tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes phosphorylated human Plk1 protein at the pT210 residue. Minimun reactivity is expected with the non-phosphorylated form of the Plk1 protein. Species sequence homology: chimpanzee, porcine, chicken, mouse, rat, Xenopus, canine, mosquito, zebra fish, starfish, sea urchin, and fruit fly.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa 205-214 of Human Polo Kinase I (Plk1).
