Applicatons:Suitable for FLISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: R-Phycoerythrin (240 kD) is a labile molecule that may dissociate into components upon exposure toreducing or denaturing agents.
Reaction with low molecular fragments is typically noted by western blot
Fluorochrome/Protein Ratio: 3.0 moles FITC per mole of Goat IgG
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Grade：Affinity Purified
Purity：Purified from monospecific antiserum by immunoaffinity chromatography using R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a lyophilized powder, pH 7.2, 10 mg/ml BSA, 0.01% sodium azide. Restore with 1.0 ml of dH2O or 40-50% glycerol for long term storage.
Specificity：Assay by IEP resulted in a single precipitin arc against anti-goat serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Isotype：IgG
Calc Applications Abbrev：FLISA IF WB
Calc Crossreactivity：Gt
Immunogen：Prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
