The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis.
Mammalian PAKs are called 1, 2, 3 or a, g, b respectively.
PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S.
cerevisiae.
Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids.
PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins.
The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution(s):ELISA: 1:5000-1:25,000; Assayed against 0.1ug of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Positive Control:Lysates from Jurkat cells or PAK transfected cells may be used as a control.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：specific for the phosphorylated form of PAK 1/2/3. Minimally reactive with the non-phosphorylated form of the immunizing peptide. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Human PAK 1/2/3 phospho-peptide corresponding to a region of the human protein conjugated to KLH. Sequence Information: PEQSKRS-(pT)-MVGTPY (aa416-429 of human PAK1, aa395-408 of human PAK2 and aa429-444 of human PAK3)
