Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form.
Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC).
Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT).
This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:60,000.
Standard capture ELISA using IgG, H&L (HRP) Pab Gt xRb and ABTS (A0030) as a substrate for 30 minutes at RT
Western Blot: 1:500- 1:2000.
Against recombinant mouse Notch constructs in 293 cells.
An 80kD band corresponding to Notch 1
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as liquid in PBS, 0.09% sodium azide. No stabilizing proteins added.
Specificity：Recognizes human NOTCH 1. Species Crossreactivity: mouse. Only the cleaved intracellular (activated) form (NICD) is detected. Species sequence homology: rat NOTCH 1-100%. The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. No reactivity is detected against mouse N2, N3 or N4.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide, aa1755-1767 VLLSRKRRRQHGQC, corresponding to human Notch 1. This is the N-terminal sequence of the cleaved N intracellular domain (NICD).
