Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form.
Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC).
Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT).
This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Applications:Suitable for use in, ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000-1:100,000 Western Blot: 1:1000-1:10,000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as liquid in PBS, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes human NOTCH 1. No crossreactivity with NOTCH 2 or Mouse Notch.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide CQHSYSSPVDNTPSHQ corresponding to amino acid residues 2488-2502 of human Notch 1, intracellular domain. A residue of cysteine was added to the amino terminal end to facilitate coupling.
