84-1072-39　NFkB, p50 (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding Subunit) 100ul　N2301-54

特徴

• NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells.
• It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IkB.
• NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits.
• Other identified subunits include p52 (NFKB2), c-Rel, and RelB.
• The p65, cRel, and RelB subunits are responsible for transactivation.
• The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate.
• p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers.
• The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a.
• IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding.
• Low levels of p52 and p50 homodimers can also exsist in cells.
• Applications:Suitable for Immunoprecipitation, Immunoblot, ELISA and Supershift assays.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:1000Western Blot: 1:1000Super shift: 0.5-1ul/assay
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.