84-1072-32　Myosin, Regulatory Light Chain of Smooth andNon-muscle, Phosphorylated pS20 100ug　M9850-02

特徴

• Myosin is the major component of thick muscle filaments, and is a long asymmetric moleculecontaining a globular head and a long tail.
• The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000-21,000 daltons.
• Activation of smoothand cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction.
• Myosin light chain phosphatase acts to regulate musclecontraction by dephosphorylating activated myosin light chain.
• This antibody is specific for the phosphorylated form of myosin light chain.
• The selected peptide sequence used to generate thepolyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes inaddition to smooth and non-muscle cells.
• This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site.
• pS20 is also referred to as pS19.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.