The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (merlin).
The protein product merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas.
Merlin's similarity to ERMs suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane.
The NF2 protein is highly expressed in human fibroblasts and detected as a single band of about 75kD.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1,2000,000 Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes human Merlin (NF2) phosphorylated at Ser518, Mr 75kD. Specific for the phosphorylated form of human Merlin (NF2). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to human Merlin, surrounding Ser518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
