84-1072-27　MAP Kinase 1 (Mitogen-activated Protein Kinase 1, MAPK 1, MAPK1, ERT1, Extracellular Signal-regulated Kinase 2, ERK2, ERK-2, ERK, MAP Kinase Isoform p42, Mitogen-activated Protein Kinase 2, MAP Kinase 2, MAPK 2, MAPK2, p41mapk, p42-MAPK, P42MAPK, PRKM1, PRKM2) 100ul　M2351-80Y

特徴

• Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses.
• In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPKs) ERK1 and ERK2.
• When activated, ERKs form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes.
• As such, Erk1 and Erk2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells.
• These enzymes function within highly conserved cascade of sequentially activating protein kinases that transducesignals from diverse extracellular stimuli.
• In vertebrates the mitogen-induced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Positive controls: U937, HeLa, NIH-3T3, RAW264.7, LNCaP and HEK whole cell lysates.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.