84-1072-25　Maltose Phosphorylase 100ug　M2155-17

特徴

• In enzymology, a maltose phosphorylase (EC 2.4.1.8) is an enzyme that catalyzes the chemical reactionmaltose + phosphate D-glucose + beta-D-glucose 1-phosphateThus, the two substrates of this enzyme are maltose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate
• This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases.
• The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase.
• This enzyme participates in starch and sucrose metabolism.
• Applications:Suitable for Immunoblotting (Western or Dot Blot), ELISA, Immunoprecipitation, Conjugation andmost immunological methods.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2000-1:10,000; 1ug of Maltose Phosphorylase was used in a standard sandwich ELISA with Rabbit anti-Goat (HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT
• Optimal dilutions to be determined by the researcher.
• *Recommended Secondary Antibodies:I1904-28K: IgG, H&L (HRP) Pab RbxGtI1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.