Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.
Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.
Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000-1:16,000Western Blot: 1:3500, band at ~42kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, ddH2O.
Aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide. Reconstitute with 1ml 40-50% glycerol, ddH2O.
Specificity：Recognizes MBP epitope tag protein at~ 42 kD.
