It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development.
LDB2 is a close homologue of LDB1.
LDBs are homologs of the Drosophila Chip protein.
Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Specificity：Recognizes mouse LDB2.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to an internal region near aa100-125 from mouse LDB2. Species sequence homology: human, chimpanzee, canine, rat and chicken
