Applications:Suitable for use in FLISA, Immunomicroscopy, Flow Cytometry, FACS, Immunofluorescence and Fluorescent Western Blot.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio:3.4 moles FITC per mole of Rabbit IgG
Excitation Wavelength: 495nm
Emission Wavelength: 528nm
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes Keyhole Limpet Hemocyanin. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, anti-fluorescein and KLH
