84-1072-17　［取扱停止］IKK epsilon, phosphorylated (Thr501) (IKKe) 100ug　I3000-62

特徴

• Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses.
• NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli.
• NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-κB activity.
• IkB proteins are phosphorylated by an IκB kinase complex consisting of at least three proteins, IKKa, IKKb andIKKγg.
• Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe.
• IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFa or IL-1.
• LPS increases the IKKe mRNA level in mouse macrophage cell lines.
• This protein hassignificant sequence homology with kinase domains of IKKa and IKKb.
• Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2500-1:5000Western Blot: 1:500Optimal dilutions to be determined by the researcher.
• Positive Control:HeLa cells or TNF inducible KBM-5 cells
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.