Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions: ELISA: 1:5000-1:50,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:200-1:1000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C as an undiluted liquid for 12 months.
Dilute only prior to immediate use.
DO NOT FREEZE.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in TBS, pH 8.0, 15mg/ml BSA ( IgG and Protease free), 0.05% sodium azide. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD)
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observedagainst anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
