Applications:Suitable for use in Western Blot, Dot Immunoblot, ELISA, Immunoelectrophoresis, Immunoperoxidase electron microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:90,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:500 to 1:5000 (tissue sections) Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute by addidng sterile 40-50% glycerol, ddH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
仕様
Size：500ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% genamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute wirh sterile 40-50% glycerol, ddH2O.
Specificity：Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-goat serum, mouse IgM and mouse serum. No reaction was observed against anti-Pepsin or anti-goat IgG F(c). No cross reactivity with other mouse heavy or light chain isotypes.
