Applications:Suitable for use in FLISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW: 390D). Reconstitute with 0.75ml sterile dH2O or 40-50% glycerol for long term storage. Absorption Wavelength: 495nm Emission Wavelength: 528nm. Fluorochrome/Protein Ratio: 2.8moles FITC per mole of Goat F(ab’)2.
Specificity:. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein,anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).