Applications:Suitable for ELISA, Western Blot and Immunohistochemistry.
Other applications not tested
Recommended Dilutions: ELISA: 1:20,000 to 1:80,000.
Assayed against 1ug of mouse IgM in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT
Western Blot: 1:2000- 1:10,000Immunohistochemistry: 1:1000- 1:5000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes mouse IgM. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgM and mouse serum. No reactivity was observed against other mouse heavy or light chain proteins
