Applications:Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 to 1:3,000, assayed against 1.0ug of Rat IgM in a standard capture using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50%Glycerol; pH 8.0, 10 mg/ml BSA (IgG and Protease free), 0.05% Sodium Azide.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine, anti-Rabbit Serum, Rat IgM, and Rat Serum. No reaction was observed against Rat IgG F(c).
