Applications:Suitable for use in Western Blot, ELISA and immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:30,000-1:70,000Western Blot: 1:1000-1;5000Immunohistochemistry: 1:500-1:2500Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Suppliied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP).
Specificity:Recognizes rat IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, rat IgM and rat serum. No reaction was observed against rat heavy or light chain proteins.